Journal: Microbial biotechnology

Article Title: Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge

doi: 10.1111/j.1751-7915.2011.00316.x

Figure Lengend Snippet: Scheme for immunization and bacterial pathogen challenge. Mice were immunized per rectum with 1 mg of EHEC O157:H7 (N°CIP 105282) BGs once (group A) or twice (group B), and with PBS once (group C) or twice (group D) serving as controls for groups A and B respectively. All mice were challenged with live heterologous EHEC O157:H7 (N°CIP 103571) on day 55. ⇓, immunization with EHEC O157:H7 (N°CIP 105282) BGs; ∇, placebo‐immunization with PBS; ↓, challenge with 1 × 10 8 live heterologous EHEC O157:H7 strain N°CIP 103571.

Article Snippet: EHEC O157:H7 BGs (N°CIP 105282) were produced in a 10 l fermenter (Medorex, Bovenden, Germany) with a stirring rate of 350 r.p.m. and 3.5 l of air per min. No antifoam was added and pH values were stable in the range 6.5–7.5.

Techniques:

Journal: Microbial biotechnology

Article Title: Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge

doi: 10.1111/j.1751-7915.2011.00316.x

Figure Lengend Snippet: Survival rate among immunized and control mice after infection with E. coli O157:H7.

Article Snippet: EHEC O157:H7 BGs (N°CIP 105282) were produced in a 10 l fermenter (Medorex, Bovenden, Germany) with a stirring rate of 350 r.p.m. and 3.5 l of air per min. No antifoam was added and pH values were stable in the range 6.5–7.5.

Techniques: Infection

Journal: Microbial biotechnology

Article Title: Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge

doi: 10.1111/j.1751-7915.2011.00316.x

Figure Lengend Snippet: Antibody titres in mice after the immunization with EHEC O157:H7 (N°CIP 105282) BGs per rectum and the heterologous oral challenge with live E. coli O157:H7 (N°CIP 103571). Each value represents the mean of antibody titres obtained from three mice: serum IgG antibody titres against EHEC O157:H7 BGs (A), serum IgA antibody titres against EHEC O157:H7 BGs (B), colon transversum IgA antibody titres against EHEC O157:H7 BGs (C), and rectal IgA antibody titres against EHEC O157:H7 BGs (D). Mice were immunized as described in Fig. 1 . Data represent the mean of three independent experiments ± SD. P ‐values < 0.05 were considered significant and are indicated with asterisks (* P < 0.05; ** P < 0.01; *** P < 0.001). a, day of the first immunization in all groups; b, day of the second immunization in groups B1 and D1; and c, day of the challenge.

Article Snippet: EHEC O157:H7 BGs (N°CIP 105282) were produced in a 10 l fermenter (Medorex, Bovenden, Germany) with a stirring rate of 350 r.p.m. and 3.5 l of air per min. No antifoam was added and pH values were stable in the range 6.5–7.5.

Techniques: BGS-A

Journal: Microbial biotechnology

Article Title: Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge

doi: 10.1111/j.1751-7915.2011.00316.x

Figure Lengend Snippet: Cellular immune responses stimulated in mice after the immunizations with EHEC O157:H7 (N°CIP 105282) BGs. Mice were immunized as described in Fig. 1 . Spleen cells (1 × 10 5 per well) were incubated for 16 h in the presence of EHEC O157:H7 BGs (specific Ag); measles inactivated Ag (strain Edmonston, non‐specific Ag) or as a negative control spleen cells were left without stimulation. The numbers of IFN‐γ‐producing cells were determined by ELISPOT assays. Rectal immunization with EHEC O157:H7 BGs effectively increased the number of IFN‐γ‐producing spleen cells after recognition of specific Ag (A). The stimulatory effect of the boost immunization was detected on day 42 (14 days after the second immunization) and remained significant until the end of experiment (B). Data represent the mean of three independent experiments ± SD (three mice per time point). P ‐values < 0.05 were considered significant and are indicated with asterisks (* P < 0.05; ** P < 0.01; *** P < 0.001). a, day of the first immunization in all groups; b, day of the second immunization in groups B1 and D1; c, day of the challenge.

Article Snippet: EHEC O157:H7 BGs (N°CIP 105282) were produced in a 10 l fermenter (Medorex, Bovenden, Germany) with a stirring rate of 350 r.p.m. and 3.5 l of air per min. No antifoam was added and pH values were stable in the range 6.5–7.5.

Techniques: Incubation, Negative Control, Enzyme-linked Immunospot

Journal: Microbial biotechnology

Article Title: Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge

doi: 10.1111/j.1751-7915.2011.00316.x

Figure Lengend Snippet: Stimulation of Ag‐specific cell proliferation after the immunizations of mice with EHEC O157:H7 (N°CIP 105282) BGs. Mice were immunized as described in Fig. 1 . Proliferation of Ag‐specific cells was assessed after 4 days of in vitro restimulation of spleen cells in the presence of EHEC O157:H7 BGs (specific Ag); measles inactivated Ag (strain Edmonston, non‐specific Ag) or as a negative control spleen cells were left without stimulation (A). The stimulatory effect of the boost immunization significantly enhanced proliferation of Ag‐specific spleen cells compared with proliferation of Ag‐specific spleen cells observed after the single immunization with EHEC O157:H7 BGs (B). Data represent the mean of three independent experiments ± SD (three mice per time point). P ‐values < 0.05 were considered significant and are indicated with asterisks (* P < 0.05; ** P < 0.01; *** P < 0.001). a, day of the first immunization in all groups; b, day of the second immunization in groups B1 and D1; c, day of the challenge.

Article Snippet: EHEC O157:H7 BGs (N°CIP 105282) were produced in a 10 l fermenter (Medorex, Bovenden, Germany) with a stirring rate of 350 r.p.m. and 3.5 l of air per min. No antifoam was added and pH values were stable in the range 6.5–7.5.

Techniques: In Vitro, Negative Control